Dear colleagues,

We are pleased to announce that the second Kiel Imaging Seminar (KIS) will take place next Monday, Nov. 28^th!

We will be introducing a new format, where authors present and discuss a recently published paper – an author’s journal club.

When: Monday, Nov. 28., 18h CET (UTC +1)

Where: https://uni-kiel.zoom.us/j/69688746633?pwd=ay92YUgxWm9DbHMvWitqOFArSUgwdz09

Who: Arianna Ferrari and Josh Peters, Kiel University

What: Author’s Journal Club on

"Performance and reproducibility of 13C and 15N hyperpolarization using a cryogen-free DNP polarizer"

Ferrari et al, Sci Rep 12, 2022, open access at https://www.nature.com/articles/s41598-022-15380-7

About the presentation:

Arianna Ferrari and Josh Peters will report on their experiences with using the SpinAlinger dissolution DNP-polarizer for ¹³C and ¹⁵N hyperpolarization. They will discuss the paper and show some new results, including now ~ 15 % ¹⁵N-polarization and fast ¹⁵N-MRI.

Abstract (from the paper):

The setup, operational procedures and performance of a cryogen‑free device for producing hyperpolarized contrast agents using dissolution dynamic nuclear polarization (dDNP) in a preclinical imaging center is described. The polarization was optimized using the solid‑state, DNP‑enhanced NMR signal to calibrate the sample position, microwave and NMR frequency and power and flip angle. The polarization of a standard formulation to yield ~ 4 mL, 60 mM 1‑13C‑pyruvic acid in an aqueous solution was quantified in five experiments to P(13C) = (38 ± 6) % (19 ± 1) s after dissolution. The monoexponential time constant of the build‑up of the solid‑state polarization was quantified to (1032 ± 22) s. We achieved a duty cycle of 1.5 h that includes sample loading, monitoring the polarization build‑up, dissolution and preparation for the next run. After injection of the contrast agent in vivo, pyruvate, pyruvate hydrate, lactate, and alanine were observed, by measuring metabolite maps. Based on this work sequence, hyperpolarized 15N urea was obtained (P(15N) = (5.6 ± 0.8) % (30± 3) s after dissolution).

About the presenters:
Arianna Ferrari is a Chemist by education and is currently pursuing her PhD in the field of hyperpolarization at Kiel University. She is part of the Research Unit FOR5042 MiTarget
Josh Peters is pursuing an MD PhD education at Kiel University. His focus is on 15N hyperpolarization and improving the polarization process.
Both are affiliated with the section biomedical imaging, clinic for radiology and neuroradiology, University Medical Center Schleswig-Holstein and Kiel University.

Please note that we intend to record and publish the session.

We are looking forward to meeting you!